Sulphosalicylic Acid Test. PDF file2To one portion add 3 parts of 3% aqueous sulphosalicylic acid Allow to stand for 10 minutes and compare with the other portion Principle And Interpretation There are two basic approaches available for measuring protein in urine the turbidimetric method and colorimetric reagent strip Sulphosalicyclic acid method comes under turbidimetric method.
Highly sensitive for Urine Protein Detects light chain proteins IV Technique Urine mixed with 20% Sulfosalicylic Acid (SSA) Variation in results may be as much as 20% Urine turbidity measured with photometer (increased turbidity with precipitated protein) Turbidity reported as trace to 4+.
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The sulfosalicylic acid (SSA) test measures urine turbidity when a small amount of aspirin is added to the urine specimen A finding of +2 to +4 suggests bacteriuria.
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Sulphosalicylic acid test If urine is alkaline it should be acidified Procedure 2ml of acidic urine taken in test tube Add an equal volume of 20% Sulphosalicylic acid Mix thoroughly allow it to stand for 10 minutes and estimate the amount of turbidity Absence of cloudiness Absence of protein If turbidity persists after boiling Positive for protein.
5Sulfosalicylic Acid an overview ScienceDirect Topics
urine protein sulfosalicylic acid precipitation test A method in which sulfosalicylic acid (that is the SSA reagent) is added to a small and equal volume of clear urine The acidification causes precipitation of protein in the sample (seen as increasing turbidity) which can be subjectively quantitated visually or more precisely quantitated using photometry.
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